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Purpose@#: This study aims to investigate the status of delirium intervention in adult intensive care unit (ICU) patients and the perception of this delirium by medical staff. @*Methods@#: This retrospective study involves 185 patients, whereas, a descriptive survey is conducted with 197 medical staff members. @*Results@#: The delirium group includes 100 patients (54.1%). The incidence of delirium is 64.9% in the medical ICU, 65.9% in the surgical ICU, 42.4% in the neuro ICU, and 46.5% in the cardiac ICU. The percentages of delirium prevention intervention differs between the two groups: 65.0% in the delirium group and 95.3% in the non-delirium group. The medical staff recognize that delirium is a common problem in the ICU (100.0%) and requires active medical intervention (98.5%). @*Conclusion@#: The length of stay at the ICU is longer in the delirium group than in the non-delirium group. It is necessary to standardize delirium prevention and treatment protocols to be equally applicable to all ICU patients.
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Purpose@#Recent studies have shown that transient receptor potential melastatin-subfamily member 7 (TRPM7) may enhance cancer cell growth, migration and invasion of human renal cell carcinoma (RCC). We investigated how TRPM7 regulated progression of RCC by interacting with matrix metalloproteinase (MMP)/tissue inhibitors of metalloproteinase (TIMP) pathway. @*Materials and Methods@#We performed a wound healing assay and a transwell migration to examine the migration of RCC cells and transwell invasion assay to assess the invasion of RCC cells. Western blot analysis was used to show the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2. @*Results@#The migration and invasion of RCC cells were markedly suppressed by siRNA targeting TRPM7. Lowering of TRPM7 increased MMP-2 expression and induced no change in MMP-9 expression. Strikingly, TRPM7 silencing suppressed the expression of TIMP-1 and TIMP-2. @*Conclusions@#These results suggested that MMP-independent action of TIMPs may take part in the enhancing effect of TRPM7 on the progression of RCC. (Korean J Urol Oncol 2020;18:61-67)
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5-HT₆ receptor (5-HT₆R) is implicated in cognitive dysfunction, mood disorder, psychosis, and eating disorders. However, despite its significant role in regulating the brain functions, regulation of 5-HT₆R at the molecular level is poorly understood. Here, using yeast two-hybrid assay, we found that human 5-HT₆R directly binds to neuro-oncological ventral antigen 1 (Nova-1), a brain-enriched splicing regulator. The interaction between 5-HT₆R and Nova-1 was confirmed using GST pull-down and co-immunoprecipitation assays in cell lines and rat brain. The splicing activity of Nova-1 was decreased upon overexpression of 5-HT₆R, which was examined by detecting the spliced intermediates of gonadotropin-releasing hormone (GnRH), a known pre-mRNA target of Nova-1, using RT-PCR. In addition, overexpression of 5-HT₆R induced the translocation of Nova-1 from the nucleus to cytoplasm, resulting in the reduced splicing activity of Nova-1. In contrast, overexpression of Nova-1 reduced the activity and the total protein levels of 5-HT₆R. Taken together, these results indicate that when the expression levels of 5-HT₆R or Nova-1 protein are not properly regulated, it may also deteriorate the function of the other.
Subject(s)
Animals , Humans , Rats , Brain , Cell Line , Cytoplasm , Eating , Gonadotropin-Releasing Hormone , Immunoprecipitation , Mood Disorders , Psychotic Disorders , RNA Precursors , RNA-Binding Proteins , Serotonin , Two-Hybrid System TechniquesABSTRACT
Scaffolds with cartilage-like environment and suitable physical properties are critical for tissue-engineered cartilage repair. In this study, decellularized porcine cartilage-derived extracellular matrix (ECM) was utilized to fabricate ECM scaffolds. Mechanically reinforced ECM scaffolds were developed by combining salt-leaching and crosslinking for cartilage repair. The developed scaffolds were investigated with respect to their physicochemical properties and their cartilage tissue formation ability. The mechanically reinforced ECM scaffold showed similar mechanical strength to that of synthetic PLGA scaffold and expressed higher levels of cartilage-specific markers compared to those expressed by the ECM scaffold prepared by simple freeze-drying. These results demonstrated that the physical properties of ECM-derived scaffolds could be influenced by fabrication method, which provides suitable environments for the growth of chondrocytes. By extension, this study suggests a promising approach of natural biomaterials in cartilage tissue engineering.
Subject(s)
Biocompatible Materials , Cartilage , Chondrocytes , Extracellular Matrix , Methods , Tissue EngineeringABSTRACT
BACKGROUND: The basophil activation test (BAT) is a promising tool for monitoring allergen-specific immunotherapy responses. OBJECTIVE: We aimed to investigate the changes in basophil activation in response to the inhalant allergens of house dust mite (HDM) and mugwort pollen during immunotherapy in patients with allergic rhinitis. METHODS: We enrolled patients with allergic rhinitis who were to receive subcutaneous immunotherapy for the inhalant allergens HDM or mugwort. A BAT was performed to assess CD63 upregulation in response to allergen stimulation using peripheral blood collected from the patients prior to immunotherapy and at 3, 6, 12, and 24 months after beginning immunotherapy. Rhinitis symptoms were evaluated using the rhinitis quality of life questionnaire (RQLQ) at 1-year intervals. RESULTS: Seventeen patients (10 with HDM sensitivity, 3 with mugwort sensitivity, and 4 with sensitivity to both HDM and mugwort) were enrolled in the study. Basophil reactivity to HDM did not change significantly during 24 months of immunotherapy. However, a significant reduction in basophil reactivity to mugwort was observed at 24-month follow-up. There was no significant association between the change in clinical symptoms by RQLQ and the change in basophil reactivity to either allergen. The change in allergen-specific basophil reactivity to HDM was well correlated with the change in nonspecific basophil activation induced by anti-FcεRI antibody, although basophil reactivity to anti-FcεRI antibody was not significantly reduced during immunotherapy. CONCLUSION: Suppression of CD63 upregulation in the BAT was only observed with mugwort at 2-year follow-up. However, the basophil response did not reflect the clinical response to immunotherapy.
Subject(s)
Humans , Allergens , Artemisia , Basophils , Desensitization, Immunologic , Dust , Follow-Up Studies , Immunotherapy , Pollen , Pyroglyphidae , Quality of Life , Rhinitis , Rhinitis, Allergic , Up-RegulationABSTRACT
PURPOSE: This study was conducted to examine the adaptation experience of male nurses working in general wards. METHODS: Experiential data was collected from 16 general ward nurses through in-depth interviews. Participants were selected from male nurses working in the general wards with over one year of clinical experience at a tertiary university hospital. The main question was “Can you describe your adaptation experience in the general ward as a nurse?” All interviews were recorded and transcribed, then analyzed using Colaizzi's method. RESULTS: Four categories were derived from the analysis: ‘difficulties from task adaptation by characteristics’, ‘limited nursing situation and circumstance as a male nurse’, ‘efforts to adapt at general nursing unit’, and ‘self-worth, and worry about something as a male nurse’. CONCLUSION: Male nurses had a difficulty adapting to work in wards due to lack of multi-tasking skills, as well as some social prejudice or negative views on male nurses. Establishing reasonable guidelines for male nurses in practice, promoting their responsibilities to patients, developing mentoring programs, and reassessing the policy of assigning male nurses to general wards are recommended to help in better adaptation.
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Humans , Male , Mentors , Methods , Nurses, Male , Nursing , Patients' Rooms , Prejudice , Qualitative ResearchABSTRACT
PURPOSE: This study was done to develop a death education program for nursing students and evaluate the effects of the program. METHODS: The education program was developed based on ADDIE model. The death education program was developed on the base of educational needs, a comprehensive review of the literature and focus group interviews and then evaluated with 53 nursing students, 27 in the experimental and 26 in the control group. Measurement was done for the meaning of life using the tool by Choi et al (2005) for attitudes concerning death, the tool Thorson and Powell (1998) revised by Kim (2006) and for attitude to end-of-life patient care, the Attitudes toward Nursing Care of the Dying Scale by Frommelt (1991) translated by Cho and Kim (2005). RESULTS: The program consisted of five sessions: Understanding of death, Family bereavement care, Communication and End-of-life patient care, Professional role, and Ethics and legal issues There was a statistically significant difference between pretest and posttest for the meaning of life in the experimental group compared to the control group. About 82% of students in the experimental group were satisfied with the program. CONCLUSION: The results indicate that this program can be used to educate nursing students.
Subject(s)
Humans , Education , Ethics , Focus Groups , Hospice Care , Nursing Care , Nursing , Patient Care , Professional Role , Students, NursingABSTRACT
BACKGROUND: Human rhinoviruses are the major cause of asthma exacerbation in both children and adults. Recently, impaired antiviral interferon (IFN) response in asthmatics has been indicated as a primary reason of the susceptibility to respiratory virus, but the mechanism of defective IFN production is little understood to date. The expression of IFN regulatory factor 7 (IRF7), a transcriptional factor for virus-induced type I IFN production is known to be regulated epigenetically by DNA methylation. OBJECTIVE: We aimed to investigate the expression of IFN-α, IFN-β, and IRF7 in response to rhinovirus infection in the adult asthmatics and evaluate DNA methylation status of IRF7 gene promotor. METHODS: Twenty symptomatic adult asthmatics and 10 healthy subjects were enrolled and peripheral blood was collected from each subject. Peripheral blood mononuclear cells (PBMCs) were isolated, cultured, and ex vivo stimulated with rhinovirus-16. The mRNA expressions of IFN-α, IFN-β, and IRF7 were analyzed using real time quantitative polymerase chain reaction. Genomic DNA was isolated from untreated PBMCs and the methylation status of IRF7 gene promotor was investigated using bisulfite pyrosequencing. RESULTS: The mean age of asthmatics was 45.4 ± 15.7 years and 40% was male, which were not different with those of control group. Asthmatics showed significantly decreased mRNA expressions (relative expression to beta-actin) of IFN-α and IFN-β compared with normal control. The mRNA expression of IRF7 in the asthmatics was also significantly lower than those in the normal control. No significant difference of DNA methylation was observed between asthmatics and controls in all analyzed positions of IRF7 promotor CpG loci. CONCLUSION: The mRNA expression of type I IFN in response to rhinovirus was impaired in the PBMCs of adult asthmatics. The mRNA expression of IRF7, transcriptional factor inducing type I IFN was also reduced, but not caused by altered DNA methylation in the IRF7 gene promotor.
Subject(s)
Adult , Child , Humans , Male , Asthma , DNA Methylation , DNA , Healthy Volunteers , Interferon Type I , Interferons , Methylation , Polymerase Chain Reaction , Rhinovirus , RNA, MessengerABSTRACT
PURPOSE: This study was conducted to comprehensively investigate nursing students' experience of their first encounter with death of a patient during clinical practice. METHODS: This study took place from January 27 through March 6, 2012 with eight female senior nursing students enrolled at Pusan National University located in Y city who have experienced patient death. We collected their experience of their first death encounter during their clinical rotation by asking, "What is your first experience of patient's death during the clinical practice?" Husserl's phenomenological approach was applied in this study. RESULTS: In this study, 17 themes, 15 clusters of themes and eight categories were derived. The categories included "Desire to avoid the reality of death", "Powerlessness", "Anticipation for recovery shifted to fear of death", "Various interpretations of death", "Limitations in their nursing practice", "Resentment of lack of nurses", "Longing to better understand death", and "Motivation for inner growth". CONCLUSION: Through their first encounter with death of a patient, nursing students experienced various emotions and viewed their role as hospice caregiver by projecting themselves as fully trained nurses in future. Participants considered terminal care as a part of nursing care. The result of this study indicates the need to include education of death in the nursing school curriculum.
Subject(s)
Female , Humans , Caregivers , Curriculum , Education , Hospices , Nursing Care , Nursing , Schools, Nursing , Students, Nursing , Terminal CareABSTRACT
Bisphenol A (BPA) has been reported to possess hepatic toxicity. We investigated the hypothesis that BPA, below the no observed adverse effect level (NOAEL), can induce hepatic damage and mitochondrial dysfunction by increasing oxidative stress in the liver. Two doses of BPA, 0.05 and 1.2 mg/kg body weight/day, were administered intraperitoneally for 5 days to mice. Both treatments impaired the structure of the hepatic mitochondria, although oxygen consumption rate and expression of the respiratory complex decreased only at the higher dose. The hepatic levels of malondialdehyde (MDA), a naturally occurring product of lipid peroxidation, increased, while the expression of glutathione peroxidase 3 (GPx3) decreased, after BPA treatment. The expression levels of proinflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) also increased. In HepG2 cells, 10 or 100 nM of BPA also decreased the oxygen consumption rate, ATP production, and the mitochondrial membrane potential. In conclusion, doses of BPA below the NOAEL induce mitochondrial dysfunction in the liver, and this is associated with an increase in oxidative stress and inflammation.
Subject(s)
Animals , Humans , Male , Mice , Adenosine Triphosphate/metabolism , Glutathione Peroxidase/metabolism , Hep G2 Cells , Inflammation/chemically induced , Injections, Intraperitoneal , Interleukin-6/metabolism , Lipid Peroxidation/drug effects , Liver/drug effects , Malondialdehyde/metabolism , Membrane Potential, Mitochondrial/drug effects , Mice, Inbred C57BL , Mitochondria/drug effects , Oxidative Stress/drug effects , Oxygen Consumption/drug effects , Phenols/toxicity , Tumor Necrosis Factor-alpha/metabolismABSTRACT
PURPOSE: This study was conducted to compare nursing students' attitudes toward death, self-esteem and life satisfaction according to clinical experience. METHODS: We surveyed 1,030 nursing students from two different universities with a questionnaire consisting of items regarding attitudes toward death, self-esteem and life satisfaction. The data were analyzed using a PASW program. RESULTS: Students with clinical experience scored 2.85 on the attitude toward death, while those without clinical experience scored 2.79 (t=2.775, P=0.006). Students with clinical experience had significantly higher self-esteem (t=4.541, P<0.001) and life satisfaction (t=4.050, P<0.001) than those without it. Students with clinical experience showed correlations between attitude toward death and self-esteem (r=0.159, P<0.001) and life satisfaction (r=0.090, P=0.025), while those without it did not (r=0.106, P=0.014). CONCLUSION: These results provide guidance for developing educational strategies such as educational program to help students build positive attitude toward death through their experience in clinical practice.
Subject(s)
Humans , Personal Satisfaction , Self Concept , Students, Nursing , Surveys and QuestionnairesABSTRACT
PURPOSE: To explore nurses' compliance with standards for critical care nursing practice in intensive care units (ICUs) and to provide basic data for high quality of critical care nursing. METHOD: A total of 616 participants from 61 ICUs which are graded from 1 to 7 throughout the nation were surveyed. Data were collected from February 9 to February 27, 2009 using a uestionnaire consisting of 58 questions including 50 nursing activities as indicators. RESULTS: The rate of ICU nurses' compliance with standards for critical care nursing practice was high. As for individual standards, compliance with the standard of assessment was the highest, followed by implementation, diagnosis, and planning in that order. There were differences in compliance according to nurses' ICU experience, work place (unit), and ICU grade. A shortage of manpower was considered as a main cause for noncompliance. CONCLUSION: The present compliance rate with standards for critical care nursing practice by ICU nurses was identified. Therefore, compliance rate can be used to promote quality of critical care nursing and development of educational programs for ICU nurses.
Subject(s)
Compliance , Critical Care , Hypogonadism , Intensive Care Units , Mitochondrial Diseases , Ophthalmoplegia , WorkplaceABSTRACT
PURPOSE: To characterize the chondrogenic potential of human mesenchymal stem cells (MSCs) in porous polymeric scaffolds by poly (glycolic acid) (PGA) as three-dimensional constructs to facilitate chondrogenic differentiation. METHODS: Human MSCs were isolated by percoll gradient method and adherent cell cultures were obtained. Isolated MSCs were characterized with CD 34 and Sca-1 antibodies using flow cytometry. MSCs were seeded in the PGA polymeric scaffolds for 28 days in a specialized defined medium. The control group was examined without the specialized defined medium. The chondrogenesis of MSCs-seeded polymer scaffolds culture was assessed by histology, RT-PCR and 35S-sulfate incorporation. RESULTS: Flow cytometry result showed that CD 34 was negative and Sca-1 was 93+/-10% positive. By the histological analysis from Safranin-O staining, it was confirmed that the chondrogenic differentiated human MSCs expressed chondrocyte-like morphologies. We also observed that type II collagen was expressed by RT-PCR. The degree of proteoglycan synthesis was higher in the experimental group than the control group. CONCLUSION: We have demonstrated that the biodegradable porous polymeric scaffolds and the specialized defined medium is able to provide three-dimensional constructs for inducing chondrogenic differentiation of human MSCs.
Subject(s)
Humans , Antibodies , Cell Culture Techniques , Chondrogenesis , Collagen Type II , Flow Cytometry , Mesenchymal Stem Cells , Polymers , ProteoglycansABSTRACT
No abstract available.